The Bruker Luxendo LCS SPIM is a compact light-sheet microscope specifically developed for large optically cleared samples. Designed with feedback from users in the lab, the LCS SPIM is easy to clean and maintain to ensure safe handling of clearing liquids. Importantly, the LCS SPIM is compatible with all currently available clearing solutions which makes it an optimal system for multi-user environments and experiments using different clearing approaches. Flexible embedding options also support small and large samples. Overall, an inverted optical concept, three objective lenses, dual-side illumination, and single-side detection ensure low sample distortion, a fast-imaging option, and an extended field-of-view (FOV). The LCS SPIM is the ideal microscope for high-throughput studies and multi-user environments.

The LCS SPIM is compatible with all currently available clearing solutions with an optical setup that supports the safe handling of samples and solutions. The benchtop design also has a Laser Safety Class 1 system.

Samples are embedded in an exchangeable quartz-crystal cuvette which comes in three different sizes. Bespoke sample holder options were also designed with users to support the stable imaging of complexly shaped objects and tissues.

Using an inverted setup with dual-side illumination, the highest quality imaging can be achieved with low sample distortion, a fast-imaging option, and an extended FOV.

When imaging, obstacles like pigments or cell nuclei can interfere with light, leading to images with shadows and striping artifacts. The next-generation SPIM employs a dual de-striping via pivot scanning and beam swiping, which generates a homogenous illumination profile without compromising acquisition speed.

Thanks to state-of-the-art technologies, ultra-fast varifocal lenses can be used to fine-tune the system's optics. Luxendo SPIMs use devices that are driven by an acoustic wave, called tuneable acoustic gradient lenses or TAG lenses. These lenses allow better light-sheet control and uniformity across the FOV.

The LCS SPIM's compact, robust and vibration-free design provides maximal stability even during long-term high-throughput experiments.

The basic configuration of the LCS offers a cost-effective solution for cleared sample imaging. Based on a very versatile layout, the highly flexible upgrade options expand the performance of the system to increase speed and optical performance.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample are affected thanks to the unique and very gentle image-acquisition concept.