MuVi SPIM LS (Live Sample)
Bruker's Multi-View Selective-Plane Illumination Microscope (SPIM) is not only the fastest light-sheet system on the market, but also incorporates years of Luxendo imaging experience and innovation. Due to its modular concept, MuVi SPIM facilitates both live-sample (LS) and cleared-sample (CS) imaging by an acquisition unit exchange.
MuVi SPIM allows for high-speed volumetric acquisition of dynamic processes in live specimens, as well as cleared sample imaging with compatibility with any clearing method.
Convenient Multiview Imaging
MuVi SPIM features a unique 4-axis concept and is available in various configurations to enable:
- Simultaneous illumination and detection from two opposing directions without rotation
- Unparalleled acquisition speed
- Free rotation axis for optimal sample positioning and 360° view
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Long-term stability for imaging time series over days
Isotropic Resolution with 360° Illumination and Detection
The MuVi SPIM is equipped with a motorized sample holder which enables sample rotation by 360°. Although sample rotation is not required to achieve in-toto imaging, a single 90° rotation of the sample can provide isotropic resolution and improve image quality. This is at least four times faster compared to conventional SPIM.
Zebrafish expressing H2A::GFP acquired simultaneously from two opposing directions (left, right). Data fusion of the two views yields a highquality in-toto image of the sample (middle).
Built on Bruker's history of innovation, our next-generation MuVi SPIM incorporates several new features to enhance image quality, shorten acquisition speed, and allow high-resolution and artifact-free imaging of a broader range of samples.
Larger Field-of-View (FOV) and Superior Axial Resolution
When imaging, obstacles like pigments or cell nuclei can interfere with light, leading to images with shadows and striping artifacts. The next-generation SPIM employs dual de-striping via pivot scanning which generates a homogenous illumination profile without compromising acquisition speed.
Improved Signal-to-Noise
When imaging thicker samples or opaque specimens, light scattering can lead to reduced contrast and image blur. MuVi SPIM has implemented an elegant approach to enhance image contrast by offering:
- Scanned light-sheet
- Adjustable light-sheet thickness
- Robust aberration tolerance (even in complex samples)
- Line illumination for improved background suppression
Dual-Destriping: Impressive Elimination of Striping Artifacts
When imaging, obstacles like pigments or cell nuclei can interfere with light, leading to images with shadows and striping artifacts. The next-generation SPIM employs dual de-striping via pivot scanning which generates a homogenous illumination profile without compromising acquisition speed.
Left: Image collected without the destriping module showing striping artifacts. Center: Image collected with the destriping module showing successful removal of striping artifacts. Right: Merged image of both the left and right image.
TAG-Lens: Light-Sheet Length Control with Fast Axial Scanning
Thanks to state-of-the-art technologies, ultra-fast varifocal lenses can be used to fine-tune the system’s optics. Luxendo SPIMs use devices that are driven by an acoustic wave, called tunable acoustic gradient lenses (or TAG lenses), for adaptive optics. These TAG lenses allow better light-sheet control and uniformity while reducing axial stretching.
